Immunoexpression of Ki-67 and p53 in Different Grades of Astrocytoma

DOI: https://doi.org/10.47648/jmsr.2023.v3502.01

Mohammad Rabiul Haque1 , Md. Zahid Hossain2 , Alimul Hasan3 , Md. Fakhrul Hassan4 , Khadiza Khanam5

Abstract

Astrocytomas are the most common intrinsic tumors of the central nervous system and derive from astrocytes.Ki-67 and p53 are two cellular proteins that have roles in pathogenesis and evolution of astrocytomas. Ki-67 is a nuclear non-histone protein produced by cells in the proliferative phases, and the Ki-67 proliferativeindex quantitatively reflects the growth potential of tumors, making it a significant biological marker. p53is a nuclear protein that controls cell cycle, apoptosis, and genomic stability. Assessment of Ki-67 and p53in various grades of astrocytoma can aid as an ancillary tool in understanding tumor behavior. This cross-sectional type of descriptive study was carried out at the Department of Pathology, RMC, Rajshahi, andBSMMU, Dhaka, over two years, between January 2021 and December 2022. In this study, a total of 30astrocytoma cases that were histologically diagnosed were included. Ki-67 and p53 immunostaining was doneon formalin-fixed paraffin-embedded tissue blocks of all cases and compared with WHO histological gradingof astrocytomas. Both biomarkers were directly associated with each other and the grade of astrocytoma.Statistically significant differences in Ki-67 LI and p53 LI were seen between grade I and grade IV andbetween grade II and IV. However, there was no statistically significant difference between grades I and II,grades I and III, or grades II and III. These markers can aid as an ancillary tool in understanding tumorbehavior and progression.

Keywords: Astrocytoma, Ki-67, p53


  1. Assistant Professor, Department of Pathology

    Holy Family Red Crescent Medical College, Dhaka

  2. Clinical Pathologist, Department of Pathology

    Rajshahi Medical College Hospital, Rajshahi

  3. Clinical Pathologist, Department of Pathology

    Rangpur Medical College Hospital, Rangpur

  4. Lecturer, Department of Pathology

    Kushtia Medical College, Kushtia

  5. Professor & Head, Department of Pathology

    Rajshahi Medical College, Rajshahi


Volume 35, Number 2 July 2023
Page: 2-9